Our findings highlight a putative role for the mitochondrial pyruvate carrier in guard cell ABA signaling in response to drought. Furthermore, transgenic lines of constitutively overexpressing NRGA1 showed opposite stomatal responses, reduced drought tolerance, and ABA sensitivity of guard cell inward K + channel inhibition and anion channel activation. The nrga1/NRGA1 functional complementation lines restored the mutant's phenotypes. Disruption of NRGA1 gene resulted in increased ABA inhibition of inward K + currents and ABA activation of slow anion currents in guard cells. The nrga1 loss-of-function mutant was very sensitive to the presence of ABA in the context of stomatal movements, and exhibited a heightened tolerance to drought stress. The heterologous co-expression of NRGA1 and AtMPC1 in yeast complemented a loss-of-function mitochondrial pyruvate carrier (MPC) mutant. for the current phylogenetic analysis by the Mega 5.2 best model prediction tool. thaliana leaf and particularly in the guard cells, and its product was directed to the mitochondria. The version of the program you are about to download is 1.11.2. K-Lite Mega Codec Pack v16.6.2 x86/圆4 - 1,724,013 Google Chrome v.45 Stable + Chromium v.0 x86/圆4 + Chrome Canary v. Statistical analyses were performed using the software SigmaPlot v11.

Here, we show that the Arabidopsis thaliana gene NRGA1 is a putative mitochondrial pyruvate carrier which negatively regulates ABA-induced guard cell signaling. Abscisic acid (ABA) regulates ion channel activity and stomatal movements in response to drought and other stresses.

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